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Analysis of an mAb (trastuzumab) and an ADC (trastuzumab emtansine) by size exclusion chromatography with online mass spectrometry (SEC-MS)
本文介紹了使用 Agilent 6530 Q-TOF 采集肽段數(shù)據(jù)， 采用整合有 BioConfirm 的 MassHunter 定性軟件，通過軟件中的蛋白酶解產(chǎn)物比較功能，實現(xiàn)了對單克隆抗體藥物中二硫鍵連接的確證。此方法無需對樣品進行額外的標(biāo)記，僅通過對還原和非還原組樣品的一級質(zhì)譜進行比對，即可實...