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Analysis of an mAb (trastuzumab) and an ADC (trastuzumab emtansine) by size exclusion chromatography with online mass spectrometry (SEC-MS)
本文介紹了使用 Agilent 6530 Q-TOF 采集肽段數(shù)據(jù)， 采用整合有 BioConfirm 的 MassHunter 定性軟件，通過(guò)軟件中的蛋白酶解產(chǎn)物比較功能，實(shí)現(xiàn)了對(duì)單克隆抗體藥物中二硫鍵連接的確證。此方法無(wú)需對(duì)樣品進(jìn)行額外的標(biāo)記，僅通過(guò)對(duì)還原和非還原組樣品的一級(jí)質(zhì)譜進(jìn)行比對(duì)，即可實(shí)...