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Streamlined workflows for N-Glycan analysis of biotherapeutics using Agilent AdvanceBio Gly-X InstantPC and 2-AB Express sample preparation with LC/FLD/MS
- Publication Part Number: 5994-1348EN
- Created: 04 Mar 2025
- 1 MB
Agilent Glycan Peak Assignment Tool for improved accuracy in N-glycan analysis with separations using the AdvanceBio Amide HILIC column.
- Publication Part Number: 5994-7477EN
- Created: 29 May 2024
- 573 KB
This user manual details how to use Agilent AdvanceBio Amide HILIC columns for analysis of N-glycans and polar analytes.
- Publication Part Number: 5994-6915EN
- Created: 14 Nov 2023
- 702 KB
In this application note, we profiled the N-glycans of several monoclonal antibodies with the new Agilent AdvanceBio Amide HILIC column.
- Publication Part Number: 5994-6916EN
- Created: 13 Nov 2023
- 980 KB
This application note presents recommended practices when using an Agilent InfinityLab Poroshell 120 HILIC-Z column in the separation of polar metabolites.
- Publication Part Number: 5994-5949EN
- Created: 22 May 2023
- 905 KB
Application compendium for glycan analysis.
- Publication Part Number: 5994-0035EN
- Created: 15 Nov 2021
- 7 MB
This collection of Agilent app notes on amino acid analysis also includes our guide on how to get accurate results with an AdvanceBio end-to-end solution.
- Publication Part Number: 5994-0033EN
- Created: 30 Apr 2021
- 4 MB
This app note demonstrates the performance of an Agilent LC/MSD to solve ambiguous peak assignments for the analysis of N-glycosylation of therapeutic proteins
- Publication Part Number: 5991-8071EN
- Created: 10 Jan 2020
- 776 KB
An Agilent AdvanceBio MS Spent Media column is used to separate saccharide and sialic acid USP standards, with an Agilent 1260 Infinity II LC and TOF MS detection.
- Publication Part Number: 5994-0320EN
- Created: 31 Oct 2018
- 718 KB
Introduction, method development, and troubleshooting of modern hydrophilic interaction chromatography (HILIC)
- Publication Part Number: 5991-9271EN
- Created: 03 May 2018
- 2 MB
Combining HILIC with MS for the analysis of underivatized amino acids in fermentation media.
- Publication Part Number: 5991-8816EN
- Created: 31 Jan 2018
- 710 KB
This application note demonstrates three approaches to quantitatively understand the glycosylation present for a given mAb.
- Publication Part Number: 5991-8796EN
- Created: 22 Jan 2018
- 1 MB
This application note demonstrates how to increase sample throughput for glycan characterization workflows using the Agilent biopharmaceutical solution.
- Publication Part Number: 5991-8550EN
- Created: 11 Oct 2017
- 4 MB
Comparison of Relative Quantification of Monoclonal Antibody N-glycans Using Fluorescence and MS Detection
- Publication Part Number: 5991-6958EN
- Created: 29 June 2016
- 2 MB
This Application Note demonstrates the sensitive and reproducible analysis of N-linked glycans of human immunoglobulin G with a novel HILIC column and fluorescence detection.
- Publication Part Number: 5991-4801EN
- Created: 01 Nov 2015
- 840 KB
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An Agilent 2.7 μm HILIC column revealed a glycopeptide not seen by RP. HILIC can be orthogonal and complementary to RP for better retention of hydrophilic peptides.
- Publication Part Number: 5991-4903EN
- Created: 19 Aug 2014
- 2 MB
The Agilent HILIC-based AdvanceBio Glycan Mapping column is 40% faster, with high resolution, efficiency, and chemical stability for at least 5,000 column volumes.
- Publication Part Number: 5991-4886EN
- Created: 30 June 2014
- 420 KB
The Agilent 1.8 μm HILIC column reduced run time by 40% when separating N-linked glycans. The column also resolved complex antennary bovine fetuin glycans.
- Publication Part Number: 5991-4896EN
- Created: 30 June 2014
- 1 MB
Comparison of two porous particles.
- Publication Part Number: 5994-1982EN
- Created: 08 Feb 2023
- 735 KB