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In this application note, we have developed a mass spectrometry-compatible, native-mode size exclusion (SEC/MS) method for characterization of a challenging FDC with very low pI.
Describes a comparison between the Agilent AdvanceBio SEC 200 ? 1.9 μm column and other vendors' SEC columns in terms of mAb and ADC analysis.
Agilent AdvanceBioSEC columns used for fast, high resolution, sensitive, and reproducible separation and quantitation of mAbs and Antibody Drug Conjugate,
Analysis of an mAb (trastuzumab) and an ADC (trastuzumab emtansine) by size exclusion chromatography with online mass spectrometry (SEC-MS)