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Clone selection is important during Monoclonal antibodies development. Proof of principle using 2D-LC/MS solution is demonstrated for identifying the correct clone.
| Korean (Korea) | Complete (PDF) |
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Agilent PLRP-S column demonstrates superior separation performance for the analysis of mAbs and ADC at both intact and fragment levels
Agilent AssayMAP Bravo platform automates a wide variety of LC/MS sample prep workflows on a single instrument including simultaneous processing of 8–96 samples.
In this paper, we are investigating an antibody drug conjugate (ADC) with a cysteine linker.
Using Microfluidic HPLC-Chip/MS
| Chinese (Simplified) (China) | Complete (PDF) |
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Using Automated Sample Preparation and LC/MS Analysis
| Chinese (Simplified) (China) | Complete (PDF) |
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In this book, we explore the steps it takes to find and evaluate an effective biosimilar, using protocals for Herceptin.
Determination of DAR for intact and reduced lysine conjugated ADCs using liquid chromatography/mass spectrometry and easy-to-use data analysis software.
| Chinese (Simplified) (China) | Complete (PDF) |
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Intact and reduced ADCs were processed using AssayMAP Bravo platform, and analyzed using the Agilent LC/MS. The DAR was determined with the Agilent DAR calculator.
| Chinese (Simplified) (China) | Complete (PDF) |
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Agilent AssayMAP Bravo automates the sample preparation for isolating surface membrane proteins in high-throughput fashion.
High sensitivity and effective interference reduction for multiple sulfur isotopes, allows accurate determination of sulfur in biological molecules using IDMS
This Application Note demonstrates the utility of capillary electrophoresis/mass spectrometry (CE/MS) for the analysis of intact monoclonal antibodies.
Combining signals from two DADs with different path length flow cells, the 1200 Infinity Series HDR-DAD Analyzer enables analysis of main and trace compounds in a single run
This work describes a high flow LCMS method to demonstrate the high resolution mass analysis of mAbs with good chromatography and its utility in analysis of Biosimilar mAbs.
| Publication Part Number | 5991-4266EN | ||
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Antibody conjugates are of significant interest in biopharma industry as therapeutic agents. This note demonstrates the use of the CE/MS analysis for immunoconjugates.
This Technical Overview describes a LC system with 3 detectors in a multi-user environment operated with Agilent MassHunter Walkup Software for LC/MS and LC systems.
Application note for Agilent Infinity Series/LC Columns Pharmaceutical Compendium
New peak modeling deconvolution (pMod) algorithm provides results with less artifacts, precise zero-charge mass measurement, and enhanced resolution of protein peaks
| Chinese (Simplified) (China) | Complete (PDF) |
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A comparison of the separation and sequence coverage of glycoproteins by reversed-phase and HILIC demonstrates the utility of HILIC.
| Publication Part Number | 5991-2085EN | ||||
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| Korean (Korea) | Complete (PDF) |
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