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Using real-time cell potency and bioenergetic assays to optimize critical process parameters for manufacturing of engineered T cells.
Using real-time cell potency and bioenergetic assays to optimize critical process parameters for manufacturing of engineered T cells with eSight and Seahorse technology.
This application note demonstrates the signal recording of the xCELLigence RTCA eSight, enabling visualization and quantification of cell migration and invasion and the speed of wound closure healing.
This application note describes a novel real-time co-culture assay using RTCA eSight for interrogating NK92 immune cell invasion and cytotoxicity and migratory kinetic profiles based on ECM and effects of TME.
Technical overview describes a method for monoclonal cell isolation using a live cell analysis system, the Agilent xCELLigence RTCA eSight, with a limiting dilution cloning method.
This application note evaluates the Agilent xCELLigence RTCA eSight as a tool to monitor BiTE antibody-mediated cancer cell lysis.
Agilent xCELLigence RTCA S16 System Data Sheet.
Agilent xCELLigence Real-Time Cell Analyzer HT-Biotek BioSpa 8 specifications data sheet.
This application note demonstrates the feasibility and use of combining multiple cell-based in vitro assays from Agilent that use diverse analytical technologies to investigate the cardiac liability of drugs.
This application note describes pulse labeling methods based on inorganic quantum dots, organic nanoparticles, dyes that covalently modify cytoplasmic proteins, and baculovirus. After evaluating each reagent’s labeling efficiency across a population...
This tech note demonstrates the ability of the RTCA eSight assay to identify and characterize antiviral drugs using adenovirus as a model system in real time.
This app note demonstrates the feasibility of using a connected workflow to evaluate the effect of inotropic compounds on electrically paced human iPSC derived cardiomyocytes (hiPSC-CMs) through calcium imaging
Measure and quantify enhanced immune cell cancer killing with antibody drug treatment; shown here is a combo of trastuzumab and PBMCs targeting HER-2 positive cells
The Agilent xCELLigence RTCA eSight monitors 3D tumor spheroid growth/shrinkage in real time using brightfield plus red, green, and blue fluorescence.
A demonstration of how the Agilent xCELLigence RTCA eSight can be used to identify and characterize virus neutralizing antibodies in real-time.
This study used the Agilent xCELLigence RTCA CardioECR system with human iPSC-derived cardiomyocytes (hiPSC-CMs) to evaluate cardiac safety/toxicity of antiviral drugs.
A demonstration of the use of the Agilent xCELLigence RTCA CardioECR system to compare functional profiles of contractility and electrophysiology between an PS-iPSC-CM disease model and its isogenic control.
Noninvasive and continuous monitoring of the perturbation of cellular growth following exposure to the cumulative toxicants present in water samples.
The xCELLigence eSight was used to continuously monitor CAR T cell-mediated killing of cancer cells.
This guide is for a basic assay using an Agilent xCELLigence RTCA instrument to monitor the effects of siRNA knockdown.