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The Agilent xCELLigence real-time cell analysis (RTCA) eSight system gives its users the flexibility to use both label-free impedance and/or imaging in four channels for the real-time monitoring of cell proliferation and cytotoxicity. The eSight has...
Here we describe image-based immune cell killing assays of 3D single tumor spheroids expressing RFP using the xCELLigence RTCA eSight.
This application note demonstrates a robust method for performing real-time antibody internalization assays using the Agilent xCELLigence RTCA eSight combined with CypHer5E pH-sensitive dyes.
This document is an information guide and checklist prepared for you that outlines the supplies, space, and utility requirements for the system set up in your lab.
In this application note, we demonstrate the utility of a cell-by-cell analysis module that uses brightfield imaging to enable label-free cell segmentation and classification based on area, eccentricity, or fluorescence intensity, allowing for...
This document details the technical specifications of the Agilent xCELLigence RTCA eSight instrument.
CAR T-cell manufacturing conditions are critical to differentiation, metabolic fitness, and cytotoxic function, enhancing therapy efficacy and production efficiency.
Poster presented at AAI 2025. Immune cell-mediated cytotoxicity of CD19 CAR-T cells against Raji suspension tumor cells was monitored and measured using real-time whole-well live cellimaging on the xCELLigence RTCA eSight system with its 5x...
In this study, we present a robust approach to monitor and analyze the bystander effect of Antibody-Drug Conjugates in real-time using live-cell imaging andimpedance measurements with the Agilent xCELLigence RTCA eSight system.
There is a need for functional potency assays that can be used to rapidly test the efficacy of different immunotherapy approaches, including but not limited tosmall molecules, biologics, cell therapies, or combinations of these agents
The eSight can be used to study cytotoxicity kinetics of lymphocytes invading a deregulated extracellular matrix present in solid tumors.
we demonstrated consistent production of CAR T-cells using the functionally closed and automated cell manufacturing Lonza Cocoon platform, using a novelcombination of cell analysis parameters obtained from an Agilent xCELLigence RTCA system, a...
The invasion of the 3D spheroid in the presence or absence of drug treatment was monitored and quantified using the brightfield and fluorescence live cell time-lapse
Cytotoxic capacity, proliferation, metabolism, phenotype and exhaustion of repeatedly challenged CAR T cells were evaluated using xCELLigence RTCA system,Seahorse XF Pro analyzer, and Novocyte flow cytometry, respectively.