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The Agilent xCELLigence real-time cell analysis (RTCA) eSight system gives its users the flexibility to use both label-free impedance and/or imaging in four channels for the real-time monitoring of cell proliferation and cytotoxicity. The eSight has...
- Publication Part Number: 5994-8429EN
- Created: 22 Sep 2025
- 2 MB
Here we describe image-based immune cell killing assays of 3D single tumor spheroids expressing RFP using the xCELLigence RTCA eSight.
- Publication Part Number: 5994-8380EN
- Created: 14 July 2025
- 2 MB
Recently updated Cancer Immunotherapy Handbook featuring the latest high impact xCELLigence publications and newly released supporting information.
- Publication Part Number: 5994-1303EN
- Created: 08 July 2025
- 6 MB
Describes use of a tethering approach to immobilize liquid tumor cell lines on to the surface of gold biosensors embedded in an Agilent E-Plate.
- Publication Part Number: 5994-1066EN
- Created: 27 June 2025
- 687 KB
This application note demonstrates a robust method for performing real-time antibody internalization assays using the Agilent xCELLigence RTCA eSight combined with CypHer5E pH-sensitive dyes.
- Publication Part Number: 5994-8106EN
- Created: 02 May 2025
- 4 MB
This document is an information guide and checklist prepared for you that outlines the supplies, space, and utility requirements for the system set up in your lab.
- Publication Part Number: 300600890
- Created: 01 Apr 2025
- 537 KB
This document is an information guide and checklist prepared for you that outlines the supplies, space, and utility requirements for the system set up in your lab.
- Publication Part Number: S2833AA
- Created: 01 Apr 2025
- 169 KB
This document is an information guide and checklist prepared for you that outlines the supplies, space, and utility requirements for the system set up in your lab.
- Publication Part Number: 380601070
- Created: 01 Apr 2025
- 481 KB
In this application note, we demonstrate the utility of a cell-by-cell analysis module that uses brightfield imaging to enable label-free cell segmentation and classification based on area, eccentricity, or fluorescence intensity, allowing for...
- Publication Part Number: 5994-8047EN
- Created: 04 Mar 2025
- 8 MB
This document details the technical specifications of the Agilent xCELLigence RTCA eSight instrument.
- Publication Part Number: 5994-1220EN
- Created: 17 Oct 2025
- 120 KB
CAR T-cell manufacturing conditions are critical to differentiation, metabolic fitness, and cytotoxic function, enhancing therapy efficacy and production efficiency.
- Publication Part Number: Cyto 2025
- Created: 05 June 2025
- 537 KB
Poster presented at AAI 2025. Immune cell-mediated cytotoxicity of CD19 CAR-T cells against Raji suspension tumor cells was monitored and measured using real-time whole-well live cellimaging on the xCELLigence RTCA eSight system with its 5x...
- Publication Part Number: AAI 2025 #3083
- Created: 14 May 2025
- 3 MB
In this study, we present a robust approach to monitor and analyze the bystander effect of Antibody-Drug Conjugates in real-time using live-cell imaging andimpedance measurements with the Agilent xCELLigence RTCA eSight system.
- Publication Part Number: AACR 2025 #5476
- Created: 25 Apr 2025
- 2 MB
AACR 2025 poster: Chronic anti-CD3/CD28 stimulation during cell expansion generates exhausted T cells with a distinct metabolic and cytotoxic phenotype
- Publication Part Number: AACR #4856
- Created: 25 Apr 2025
- 475 KB
This poster aims to demonstrate the capability of impedancebased xCELLigence RTCA assays for virus and antibody titer, as well as for identification and characterization of antiviral drugs.
- Created: 25 Apr 2025
- 930 KB
The poster introduces a multiparametric workflow that allows for evaluating contractility, viability, and metabolic function using validated human iPSC-derived cardiomyocytes to better understand the mechanisms associated with drug-induced...
- Publication Part Number: SOT # 4373
- Created: 24 Mar 2025
- 504 KB
This study aims to optimize and validate a drug safety testing workflow that integrates mitochondrial toxicity assessment using Seahorse XF analysis solution with cell viability assessment via the xCELLigence RTCA platform.
- Created: 19 Mar 2025
- 437 KB
There is a need for functional potency assays that can be used to rapidly test the efficacy of different immunotherapy approaches, including but not limited tosmall molecules, biologics, cell therapies, or combinations of these agents
- Publication Part Number: #91
- Created: 19 Mar 2025
- 2 MB
The eSight can be used to study cytotoxicity kinetics of lymphocytes invading a deregulated extracellular matrix present in solid tumors.
- Created: 19 Mar 2025
- 2 MB
This quick reference guide provides detailed instructions on how to prepare, perform, and evaluate the impedance-based TCID50 protocol using the xCELLigence RTCA Software Pro Virology module to determine viral titer
- Publication Part Number: 5994-7860EN
- Created: 18 Mar 2025
- 143 KB