PLRP-S HPLC 柱全新可持續(xù)包裝
用于生物分子 HPLC 的 PLRP-S 色譜柱
聚苯乙烯/二乙烯基苯 (PS/DVB) 顆粒填料固有的疏水化學(xué)性質(zhì)意味著無需反相分離所需要的鍵合相,這使其成為一種高重現(xiàn)性的材料,同時不含硅醇基和重金屬離子。我們廣泛的產(chǎn)品系列包括適用于分析級分離和制備純化的色譜柱。 其具有出色的熱穩(wěn)定性和化學(xué)穩(wěn)定性,尤為適合需要在極端條件下進行樣品前處理、化合物洗脫和色譜柱再生的純化應(yīng)用。
PLRP-S 具有多種填料孔徑可供選擇,且操作條件范圍更寬,能夠為實現(xiàn)理想分離提供更多選擇。填料粒徑范圍從 3 μm 到 50 μm,適用于從研發(fā)階段的 μg/mg 水平放大到 cGMP 生產(chǎn)的幾千克級水平。卓越的化學(xué)穩(wěn)定性,NaOH 濃度可高達 1 mol/L,可通過沖洗和再生延長色譜柱壽命。PLRP-S 填料批次規(guī)??筛哌_ 600 L,多根色譜柱可采用同一批次填料進行裝填。
- Intact & Subunit Analysis
Product Details
- 具有耐用可靠的聚合物顆粒填料,能在更長的使用壽命內(nèi)提供高重現(xiàn)性結(jié)果
- 分離、優(yōu)化、清洗和再生的優(yōu)良熱穩(wěn)定性和化學(xué)穩(wěn)定性,提高了選擇性和柱壽命
- 遵循 USP L21 標(biāo)準(zhǔn)
- 填料粒徑范圍從 3 μm 到 50 μm
- 各種孔徑 (100 ?-4000 ?),適用于從小分子到大分子復(fù)合物、蛋白質(zhì)、多肽和多聚核苷酸的分離
Videos
- Application Notes
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An In-Depth Analysis of Semaglutide, a Glucagon-Like Peptide-1 Receptor Agonist
In this application note, we explore some of the key parameters when comparing different column chemistries using ion-pair reversed-phase (IP-RP) separation of Semaglutide.
- Application Notes
- English
- 10 May 2024
- 529.96 KB
Agilent Biocolumns Application Compendium - Intact and Subunit Purity
Application compendium for intact and subunit purity
- Application Notes
- English
- 28 Jun 2021
- 3.08 MB
Precise Characterization of Intact mAbs by the Agilent 6545XT AdvanceBio LC/Q-TOF
This app note presents a complete high-throughput workflow solution to analyze a variety of mAb products.
- Application Notes
- English
- 19 Nov 2018
- 1.31 MB
An Integrated Workflow for Intact and Subunits of Monoclonal Antibody Accurate Mass Measurements
An Integrated Workflow for Intact and Subunits of Monoclonal Antibody Accurate Mass Measurements
- Application Notes
- English
- 29 Mar 2018
- 13.65 MB
Measuring Drug-to-Antibody Ratio (DAR) for Antibody-Drug Conjugates(ADCs) with UHPLC/Q-TOF
In this paper, we are investigating an antibody drug conjugate (ADC) with a cysteine linker.
- Application Notes
- English
- 13 Oct 2016
- 1.15 MB
PLRP-S Polymeric Reverse Phase Column for LC/MS Separation of mAbs and ADC
Agilent PLRP-S column demonstrates superior separation performance for the analysis of mAbs and ADC at both intact and fragment levels
- Application Notes
- English
- 01 Aug 2016
- 287.58 KB
Improved Column Lifetime with Thermally Stable Polymer Columns for Oligonucleotide Ion-Pair RP HPLC
Evaluation of the thermal stability and lifetime of the columns subjected to 100 cycles of 25-minute long gradient cycles at either 80 °C (PLRP-S) or 60 °C (all other columns)
- Application Notes
- English
- 27 Mar 2013
- 1003.98 KB
High performance DNA oligonucleotide purification using Agilent TOP-DNA
Because of the significant progress achieved in the solid phase chemical synthesis of oligonucleotides during the past four decades, synthetic oligonucleotides...
- Application Notes
- English
- 26 Sep 2012
- 707.71 KB
- Brochures
- Catalogs
- Support
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Agilent BioHPLC and AdvanceBio Reversed-Phase Columns User Guide
This user guide provides general information for the Agilent BioHPLC and AdvanceBio reversed-phase columns.
- User Manuals
- English
- 28 Oct 2020
- 1007.45 KB
Agilent PLRP-S Bulk Media Packing
This user guide describes the preparation of the slurry and column packing, column testing, and ordering information for the Agilent PLRP-S bulk media.
- User Manuals
- English
- 07 Aug 2025
- 253.44 KB
- FAQs
優(yōu)化合成肽的分析與純化
包括 GLP-1 RA 在內(nèi)的多肽治療藥物,通常使用固相多肽合成生產(chǎn)。這種按順序添加氨基酸的多步驟工藝可能會引入各種工藝相關(guān)雜質(zhì)。在分析和制備規(guī)模實現(xiàn)高純度和高產(chǎn)量對于高效的 GLP-1 工作流程至關(guān)重要。PLRP-S 色譜柱和散裝填料具有優(yōu)化的孔徑和易擴展特性,是 GLP-1 純化的理想選擇,非常適合開發(fā)和生產(chǎn)環(huán)境。
- 01 Jan 2025