
Seahorse XFe24 Analyzer
OCR and ECAR rates are key indicators of mitochondrial respiration and glycolysis as well as ATP production rate, and together these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex-vivo samples.
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Product Details
- Live cell, real-time analysis of cellular energy metabolism platform in 24-well format
- Multiple parameters reported from each assay well, including OCR, PER or ECAR, and ATP product rates
- Large well size and transient microchamber to provide the capacity for larger and/or more metabolically active samples
- Robust response with as few as 10,000 cells per well in the custom 24-well plate
- Compatible with 3D study models such as islets and small organism (for example, zebrafish)
- Four-port injection system with automated mixing feature for assessing immediate cellular responses to substrates, inhibitors, and other compounds in real time
- Wide operation environment temperature of 4 to 30 °C, allowing the analyzer to maintain internal assay temperature of 16 to 42 °C for compatibility with a variety of samples
- Data files compatible with the web-based analytics tool, Seahorse Analytics
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Automatic measurement of energy metabolism in real time
Agilent Seahorse XF Analyzers measure oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a multi-well plate, interrogating key cellular functions such as mitochondrial respiration and glycolysis. Seahorse XF Analyzers perform compound addition and mixing, label-free analytical detection, and automatic measurement of OCR and ECAR in real time.
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Measuring functional metabolism of brite adipocytes
Mitochondrial function correlates to brown adipocyte function
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The Agilent Seahorse XF Pro platform
Transforming Bioenergetics - Again. Featuring new pharma-oriented workflow solutions with improved precision, along with advanced experimental design and analysis tools, you can optimize your workflow from start to finish.
- 17 Jan 2022
The Seahorse XF Flex Analyzer: Powerful Metabolic Insights in 2D & 3D Models
The 24-well Agilent XF Flex Analyzer offers enhanced sensitivity, a broad detection range, and compatibility with 3D models like organoids, tissues, and co-cultures. Purpose-built consumables and powerful analysis tools help you design smarter experiments, extract deeper insights, and generate translatable data—empowering your research to reach new heights in cellular metabolism and function.
- 14 Apr 2025
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Real-time Cell Metabolic Analysis – FAQs
Discover answers to common questions about real-time cell metabolic analysis, focusing on Agilent Seahorse XF systems.
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