
RTCA E-Plates
The xCELLigence RTCA system captures dynamic cell responses from seconds to days, providing rich, time-resolved data. E-Plates are available in 16-, 48-, 96-, and 384-well formats, offering flexibility for various throughput needs. Select E-Plates also feature a biosensor-free viewing window, allowing for complementary imaging without interference.
- RTCA Microplates
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Product Details
- Observe the cell response in real-time
- Derive cell kinetics
- Obtain continuous immune cell killing assay time points during the entire experiment
- RTCA provides a continuous quantitative readout of proliferation rate, cell number, size/shape, and substrate attachment quality
Cellular Impedance Explained
The functional unit of a cellular impedance assay is a set of gold microelectrodes fused to the bottom surface of a microtiter plate well. When submersed in an electrically conductive solution (such as buffer or standard tissue culture medium), the application of an electric potential across these electrodes causes electrons to exit the negative terminal, pass through bulk solution, and then deposit onto the positive terminal to complete the circuit.
Learn More- Application Notes
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xCELLigence Portfolio Cancer Immunotherapy Handbook
Recently updated Cancer Immunotherapy Handbook featuring the latest high impact xCELLigence publications and newly released supporting information.
- Application Notes
- English
- 08 Jul 2025
- 6.04 MB
Evaluating Functional Potency of Immunotherapies Targeting Liquid Tumors
Describes use of a tethering approach to immobilize liquid tumor cell lines on to the surface of gold biosensors embedded in an Agilent E-Plate.
- Application Notes
- English
- 27 Jun 2025
- 686.86 KB
Vaccine and Virology Applications
A collection of studies showing the benefits of xCELLigence RTCA assays over traditional assays. Antiviral drugs, virucides, neutralizing antibodies and other applications are examined.
- Application Notes
- English
- 02 Jul 2020
- 4.37 MB
Infectious Disease Applications
A compilation of studies demonstrating the application of xCELLigence RTCA to the analysis of bacterial toxins, biofilms, virus titer determination, and other areas of disease.
- Application Notes
- English
- 02 Jul 2020
- 4.93 MB
Real-Time Quality Control and Functional Assessment of Mesenchymal Stem Cells for Cellular Therapies
An xCELLigence RTCA was used to develop a label-free assay to functionally assess BMSCs.
- Application Notes
- English
- 02 Jul 2020
- 773.17 KB
Studying Bacterial Biofilms Using Cellular Impedance
Describes the use of Agilent xCELLigence real-time cell analysis (RTCA) instruments for impedance monitoring.
- Application Notes
- English
- 02 Jul 2020
- 1.94 MB
Identifying and Characterizing Endocrine Disruptors Using a Cell Panel-based Real-time Assay
Describes a cell-based assay for quantitative, noninvasive, and continuous monitoring of cellular responses to chemicals of interest, using an Agilent xCELLigence real-time cell analysis (RTCA) instrument.
- Application Notes
- English
- 02 Jul 2020
- 950.13 KB
Real-Time, Label-Free Measurement of Natural Killer Cell Activity and Antibody-Dependent Cell-Mediated Cytotoxicity
Explores how the Agilent xCELLigence system can be used for the dynamic real-time monitoring of cell-mediated cytotoxicity and the impact of specific antibodies.
- Application Notes
- English
- 02 Jul 2020
- 1.53 MB
Agilent xCELLigence Real-Time Cell Analysis
A demonstration of the ways in which virus-mediated cytopathogenicity can be monitored using an Agilent xCELLigence RTCA instrument.
- Application Notes
- English
- 02 Jul 2020
- 387.24 KB
Tumor Cell Killing by T Cells
An assay workflow demonstration measuring cytotoxic T lymphocyte-mediated destruction of tumor cells and the corresponding secretion of cytokines using an Agilent xCELLigence and NovoCyte flow cytometer instrument.
- Application Notes
- English
- 02 Jul 2020
- 601.08 KB
- Data Sheets
Agilent Cell Analysis Publication Database
Search scientific publications by research area, cell type and more.
Agilent Cell Analysis Webinars
Featured webinars help you stay informed about timely cell analysis research topics.
Videos
Agilent xCELLigence RTCA Instrumentation Supports Potency Assay Development in Recent FDA Approval
Agilent xCELLigence Real-Time Cell Analysis (RTCA) technology supports the testing of accuracy and reliability in AUCATZYL, a recently approved CAR T therapy by Autolus Therapeutics. The xCELLigence RTCA technology supported the development and implementation of the potency assay. By providing precise and reliable cell analysis capabilities, Agilent supported Autolus's efforts in establishing robust analytical methods.
- 15 Jul 2025