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An Agilent 2.7 μm HILIC column revealed a glycopeptide not seen by RP. HILIC can be orthogonal and complementary to RP for better retention of hydrophilic peptides.
This Application Note shows the workflow solution for the physicochemical and biological characterization of a biosimilar and innovator rituximab.
The charge heterogeneity provides a great challenge for the complete characterization of the monoclonal antibody (mAb).
Fast and efficient LC TOF/MS characterizes intact and digested IgG to identify glycoform masses and profiles. mAb-glyco chip analysis built a glycan accurate mass database.
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