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This application note describes multi-attribute analysis of monoclonal antibodies using the Agilent InfinityLab 2D-LC Solution and the Agilent 6530 LC/Q-TOF system.
"This Application Note describes the quantification of aggregates in biotherapeutic
Small –scale purification of a monoclonal antibody from a cell lysate and the subsequent analysis on a single instrument, the Agilent 1260 Infinity Bio-inert Quaternary LC.
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Agilent Bio-Monolith Protein A delivers high-throughput, precise, accurate, linear, and robust separation of mAbs to optimize clone selection and cell cultures.
A versatile Agilent Bio-Monolith Protein A column analyzed an optimized recombinant mAb manufactured with desired structural characteristics.
An Agilent Bio-Monolith Protein A column determined recombinant mAb titer and was used to enrich μg amounts of antibody for further structural characterization by MS.