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This application note describes multi-attribute analysis of monoclonal antibodies using the Agilent InfinityLab 2D-LC Solution and the Agilent 6530 LC/Q-TOF system.
The evaluation of different salt gradients was performed on the Agilent 1290 Infinity II Bio LC System and analyzed for resolution as well as reproducibility.
Method ransfer from a 1260 Infinity Bio-inert LC to a 1260 Infinity II Bio-inert LC showing proof of equivalency for charge variant analysis of rituximab
Small –scale purification of a monoclonal antibody from a cell lysate and the subsequent analysis on a single instrument, the Agilent 1260 Infinity Bio-inert Quaternary LC.
The Agilent 1260 Infinity Bio-inert Quaternary LC with fluorescence detection and Agilent Bio SAX analyzed N-linked glycans with.excellent resolution and precision.
Analysis of complex N-linked glycans in erythropoietin using comprehensive and multiple heart-cutting techniques with the Agilent 1290 Infinity 2D-LC solution
Agilent Bio Mab WCX separates charged variants of an intact monoclonal antibody, followed by characterization with reversed-phase chromatography and UV/MS detection.
Agilent Bio Mab WCX separates charged variants of papain-digest monoclonal antibody, following by MS characterization of the charged variants with online desalting.
This Application Note describes a high-resolution, pH-based separation of acidic and basic charge variants for monoclonal antibodies using the Agilent 1260 and BiomAb PEEK
Agilent applications chemists share their recommendations for an optimum LC system and its configuration for characterizing biomolecules. They also offer guidance on a generic method to get you started.