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The Agilent AdvanceBio SEC column together with the Agilent 1260 Infinity II Bio-inert System is an ideal combination for the aggregate analysis of ADCs
Identification of monoclonal antibody mutation sites using the 1290 Infinity II 2D-LC solution with 6530 Q-TOF LC/MS.
An SEC method identifying innovator and biosimilar insulin analog was developed using an Agilent AdvanceBio SEC 130?, 7.8 × 300 mm, 2.7 μm column.
Comparison of HPLC method and a quick method developed using the Cary 60 UV-Vis
Size Exclusion Chromatography Method Development of NIST mAb Using an Agilent AdvanceBio SEC 200 ?, 1.9 μm Column
Describes a comparison between the Agilent AdvanceBio SEC 200 ? 1.9 μm column and other vendors' SEC columns in terms of mAb and ADC analysis.
Size exclusion chromatography with light scattering detection was used to investigate the impact of varying SDS concentration on protein molecules.
An aggregate analysis workflow for SEC mobile phase optimization and characterization of mAb aggregation profiles
"This Application Note describes the quantification of aggregates in biotherapeutic
Online 2D-LC characterization of monoclonal antibodies using SEC in the first dimension and IEX in the second dimension
A SEC method for determining the purity of PEG Granulocyte Colony Stimulating Factor using an Agilent AdvanceBio SEC column with aqueous mobile phase.
"Agilent AdvanceBio SEC columns have been developed to provide optimum performance regardless of the instrument used.
Shorter 150 mm AdvanceBio SEC columns and higher flow rates of 1.5 mL/min increase sample throughput by 400%, from 1.3 days to under 8 hours.
Agilent AdvanceBio SEC columns perform better in resolution, sensitivity, peak capacity and shape of mAbs and ADCs using low pressure LC and aqueous mobile phase.
Agilent AdvanceBio SEC Columns. End Uncertainty in Monoclonal Antibody Characterization.
Analysis of an mAb (trastuzumab) and an ADC (trastuzumab emtansine) by size exclusion chromatography with online mass spectrometry (SEC-MS)
High resolution and sensitive size exclusion chromatography separates and quantitates rituximab aggregates/degradants in a bioinert LC with an AdvanceBio SEC column.
Methods are given for the use of Agilent Bio-SEC columns, with LS, UV and RI detection, for quantification or quality control of protein aggregates
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Simple, sensitive SEC quantitates aggregates in biotherapeutic mAb and ADC with the same aqueous eluent, without organic, on a bioinert LC and AdvanceBio SEC column.