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Presents the use of AdvanceBio Desalting-RP cartridges to pretreat mAb and ADC samples before MS analysis.
Agilent Bio Mab WCX separates charged variants of papain-digest monoclonal antibody, following by MS characterization of the charged variants with online desalting.
An Agilent Bio-Monolith Protein A column determined recombinant mAb titer and was used to enrich μg amounts of antibody for further structural characterization by MS.