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Agilent-NIST mAb application compendium
The development and manufacturing of biosimilars require comparability studies with innovator biotherapeutic products.
Small –scale purification of a monoclonal antibody from a cell lysate and the subsequent analysis on a single instrument, the Agilent 1260 Infinity Bio-inert Quaternary LC.
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Agilent Bio Mab WCX separates charged variants of papain-digest monoclonal antibody, following by MS characterization of the charged variants with online desalting.
Ion exchange is a commonly used technique for the separation of complex protein mixtures.
| Publication Part Number | 5990-9931EN | ||||
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Explains IEX chromatography, column selection, mobile phase considerations, general rules of thumb for using IEX, and more.