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This user manual details how to use Agilent AdvanceBio Amide HILIC columns for analysis of N-glycans and polar analytes.
- Publication Part Number: 5994-6915EN
- Created: 14 Nov 2023
- 702 KB
In this application note, we profiled the N-glycans of several monoclonal antibodies with the new Agilent AdvanceBio Amide HILIC column.
- Publication Part Number: 5994-6916EN
- Created: 13 Nov 2023
- 980 KB
A guide to find the right column for your characterization workflow. It includes advice and tips on method development, solvent choice, mobile phase modification, optimization, example separations, as well as ordering information.
- Publication Part Number: 5994-6123EN
- Created: 01 Oct 2023
- 36 MB
Push your Glycoprotein analysis to new levels of performance.
- Publication Part Number: 5994-1647EN
- Created: 11 Mar 2022
- 7 MB
Application compendium for glycan analysis.
- Publication Part Number: 5994-0035EN
- Created: 15 Nov 2021
- 7 MB
This app note demonstrates the performance of an Agilent LC/MSD to solve ambiguous peak assignments for the analysis of N-glycosylation of therapeutic proteins
- Publication Part Number: 5991-8071EN
- Created: 10 Jan 2020
- 776 KB
This application note demonstrates three approaches to quantitatively understand the glycosylation present for a given mAb.
- Publication Part Number: 5991-8796EN
- Created: 22 Jan 2018
- 1 MB
This application note demonstrates how to increase sample throughput for glycan characterization workflows using the Agilent biopharmaceutical solution.
- Publication Part Number: 5991-8550EN
- Created: 11 Oct 2017
- 4 MB
Comparison of Relative Quantification of Monoclonal Antibody N-glycans Using Fluorescence and MS Detection
- Publication Part Number: 5991-6958EN
- Created: 29 June 2016
- 2 MB
This Application Note demonstrates the sensitive and reproducible analysis of N-linked glycans of human immunoglobulin G with a novel HILIC column and fluorescence detection.
- Publication Part Number: 5991-4801EN
- Created: 01 Nov 2015
- 840 KB
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An Agilent 2.7 μm HILIC column revealed a glycopeptide not seen by RP. HILIC can be orthogonal and complementary to RP for better retention of hydrophilic peptides.
- Publication Part Number: 5991-4903EN
- Created: 19 Aug 2014
- 2 MB
The Agilent HILIC-based AdvanceBio Glycan Mapping column is 40% faster, with high resolution, efficiency, and chemical stability for at least 5,000 column volumes.
- Publication Part Number: 5991-4886EN
- Created: 30 June 2014
- 420 KB
The Agilent 1.8 μm HILIC column reduced run time by 40% when separating N-linked glycans. The column also resolved complex antennary bovine fetuin glycans.
- Publication Part Number: 5991-4896EN
- Created: 30 June 2014
- 1 MB