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A guide to find the right column for your characterization workflow. It includes advice and tips on method development, solvent choice, mobile phase modification, optimization, example separations, as well as ordering information.
This application note tests the recombinant protein A column at the maximum flow rate and performs the bridging study against the native protein A column.
Agilent Bio-Monolith Protein G quantitates supernatant mAbs across a wide loading range at many flow rates, complementing Protein A for fast titer analysis.
This application note describes multi-attribute analysis of monoclonal antibodies using the Agilent InfinityLab 2D-LC Solution and the Agilent 6530 LC/Q-TOF system.
Small –scale purification of a monoclonal antibody from a cell lysate and the subsequent analysis on a single instrument, the Agilent 1260 Infinity Bio-inert Quaternary LC.
Agilent Bio-Monolith Protein A delivers high-throughput, precise, accurate, linear, and robust separation of mAbs to optimize clone selection and cell cultures.
An Agilent Bio-Monolith Protein A column determined recombinant mAb titer and was used to enrich μg amounts of antibody for further structural characterization by MS.
This Application Note describes the quantification of human IgG with the Agilent Bio-Monolith Protein A HPLC Column with alternating column regeneration.
In this application note, pre-packed Bio-monolith Protein A columns, part of the AdvanceBio family, were used to demonstrate the quick capture of monoclonal antibody titer