Refine Results
- Applications and Industries
- Content Type
- Analysis Technique
- Analyzed With
- Chemical Group
- Compounds
- Detection Technique
- Injection Technique
- Instrument
- Analysis Platform
- Language
1 - 18 of 18 results
A guide to find the right column for your characterization workflow. It includes advice and tips on method development, solvent choice, mobile phase modification, optimization, example separations, as well as ordering information.
This application note describes multi-attribute analysis of monoclonal antibodies using the Agilent InfinityLab 2D-LC Solution and the Agilent 6530 LC/Q-TOF system.
Critical Quality Attributes Charge Variant compendium
the Agilent Buffer Advisor software in combination with the Agilent 1260 Infinity Bio-inert Quaternary LC System is an ideal solution for automated protein separation
The evaluation of different salt gradients was performed on the Agilent 1290 Infinity II Bio LC System and analyzed for resolution as well as reproducibility.
The development and manufacturing of biosimilars require comparability studies with innovator biotherapeutic products.
Method ransfer from a 1260 Infinity Bio-inert LC to a 1260 Infinity II Bio-inert LC showing proof of equivalency for charge variant analysis of rituximab
AdvanceBio Desalting - RP cartridges used for effective desalting of LC-separated monoclonal antibodies (mAbs) before characterization by mass spectrometry
Small –scale purification of a monoclonal antibody from a cell lysate and the subsequent analysis on a single instrument, the Agilent 1260 Infinity Bio-inert Quaternary LC.
| Publication Part Number | 5991-1195EN | ||||
|---|---|---|---|---|---|
| Analysis | Analysed with Part No. |
|
|||
| Instrument |
|
||||
| Analytical Technique | Technique |
|
|||
| Injection |
|
||||
| Detection |
|
||||
| Sample | Chemical Group |
|
|||
| Sample Matrix |
|
Now you have more columns and more choices to improve your charged-variant analysis of proteins with the Agilent Ion-exchange BioHPLC columns.
Agilent Bio Mab WCX separates charged variants of an intact monoclonal antibody, followed by characterization with reversed-phase chromatography and UV/MS detection.
Agilent Bio Mab WCX separates charged variants of papain-digest monoclonal antibody, following by MS characterization of the charged variants with online desalting.
Agilent Bio MAb columns, in an Agilent 1260 Infinity Bio-inert Quaternary LC, characterize the charge heterogeneity of IgG1monoclonal antibody...
| Publication Part Number | 5991-0895EN | |||
|---|---|---|---|---|
| Analysis | Analysed with Part No. |
|
||
| Instrument |
|
|||
| Analytical Technique | Technique |
|
||
| Injection |
|
|||
| Detection |
|
|||
| Sample | Chemical Group |
|
||
| Sample Matrix |
|
Ion exchange is a very useful technique to separate protein mixtures under mild conditions, most commonly by applying a shallow gradient of increasing salt concentration as el
| Publication Part Number | 5990-9629EN | |||
|---|---|---|---|---|
| Analysis | Analysed with Part No. |
|
||
| Instrument |
|
|||
| Analytical Technique | Technique |
|
||
| Injection |
|
|||
| Detection |
|
|||
| Sample | Chemical Group |
|
||
| Sample Matrix |
|
This Application Note describes a high-resolution, pH-based separation of acidic and basic charge variants for monoclonal antibodies using the Agilent 1260 and BiomAb PEEK
| Publication Part Number | 5991-1407EN | ||
|---|---|---|---|
| Analysis | Analysed with Part No. |
|
|
| Instrument |
|
||
| Analytical Technique | Technique |
|
|
| Injection |
|
||
| Detection |
|
||
| Sample | Chemical Group |
|
|
| Sample Matrix |
|
Agilent applications chemists share their recommendations for an optimum LC system and its configuration for characterizing biomolecules. They also offer guidance on a generic method to get you started.
With a completely metal free sample flow path, there's no longer any need to tolerate metal interactions or hardware absorption of your precious sample.
Explains IEX chromatography, column selection, mobile phase considerations, general rules of thumb for using IEX, and more.