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In this application note, we profiled the N-glycans of several monoclonal antibodies with the new Agilent AdvanceBio Amide HILIC column.
An Agilent 2.7 μm HILIC column revealed a glycopeptide not seen by RP. HILIC can be orthogonal and complementary to RP for better retention of hydrophilic peptides.
The Agilent HILIC-based AdvanceBio Glycan Mapping column is 40% faster, with high resolution, efficiency, and chemical stability for at least 5,000 column volumes.
The Agilent 1.8 μm HILIC column reduced run time by 40% when separating N-linked glycans. The column also resolved complex antennary bovine fetuin glycans.