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Accurate mass intact protein analysis using 6550 iFunnel Q-TOF
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采用微流控 HPLC-Chip/MS 技術(shù)
分析抗體-藥物偶聯(lián)物的藥物結(jié)合位點——利用自動化樣品前處理與 LC/MS 分析
本研究描述了使用先進液相色譜/質(zhì)譜聯(lián)用技術(shù)與簡單易用的數(shù)據(jù)分析軟件測定完整/還原態(tài)賴氨酸偶聯(lián) ADC 的 DAR。
本應(yīng)用簡報中采用 Agilent AssayMAPBravo 平臺對經(jīng)/未經(jīng)去糖基化的完整和還原態(tài) ADC 進行了平行處理，并采用安捷倫LC/MS 對其進行分析，隨后通過安捷倫DAR 計算器確定 DAR。