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Describes the use of the Agilent ChemStation Method Scouting Wizard in method development to separate and detect active compounds using SFC and TOF MS.
A reducing online 2D-LC/TOF MS method was developed to measure antibody heavy chain glycoforms directly from a bioreactor. The measurement was used in a PID control loop to control the glycoforms during a 30 day bioreactor run.
The method discussed here is useful in the quantitation of limonin in foods, food additives, juices and plants. The use of the Agilent LC/ToFMS allows for excellent quantitation of limonin as well as the characterization of other congeners.